HSP 70kDa produced in E. coli is a single, non-glycosylated polypeptide chain (1-641 a.a.) containing 661 amino acids fused to a 20 a.a. His-tag at N-terminus and having a total Mw of 72.2 kDa.
Function:
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes thro
Cell localization:
Cytoplasm
Purification:
Greater than 95.0% as determined by SDS-PAGE.
Storage conditions:
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
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