| Supplier: | Boster |
|---|---|
| Reference: | P00024-6 |
| Name: | Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody, Clone#RM251 |
| Category: | Antibody |
| Subcategory: | Primary Antibodies,Monoclonal Antibodies |
| Research Area: | Apoptosis,Cancer,Cell Cycle,Cell Death,Epigenetics and Nuclear Signaling,Metabolism,Metabolism Processes,Nuclear,Pathways and Processes,Protein Phosphorylation,Ser/Thr Kinases,Serine/Threonine Kinases,Signal Transduction |
| Aditional Names: | RAC-alpha serine/threonine-protein kinase;2.7.11.1;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha;AKT1;PKB, RAC; |
| Unit: | 100ul |
| Concentration: | 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Clonality: | Monoclonal |
| Price: | Consultar |
| Format: | Liquid |
| Immunogen: | A phospho-peptide corresponding to human Phospho-Akt |
| Host: | Rabbit |
| Reactivity: | Human |
| Cross Reactivity: | This antibody reacts to Akt only when phosphorylated at Ser473. There is no cross-reactivity with Akt without phosphorylation at Ser473. This antibody may also react to bovine, mouse or rat Phospho-Akt (Ser473), as predicted by immunogen homology |
| Application: | IHC,WB |
| Description: | Boster Bio Anti-Phospho-Akt (Ser473) AKT1 Rabbit Monoclonal Antibody, Clone#RM251 (Catalog # P00024-6). Tested in IHC, WB applications. This antibody reacts with Human. |
| Function: | AKT1 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine |
| Cell localization: | Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where |
| Clone: | Clone: RM251 |
| Isotype: | Rabbit IgG |
| Purification: | Protein A affinity purified from an animal origin-free culture supernatant |
| Storage conditions: | Store at -20°C for one year. Avoid repeated freeze-thaw cycles. |
| Assay inforation: | Immunohistochemistry (IHC): 1:200-1:500 dilution WB: 1:1000-1:2000 dilution. |