Supplier: | Boster |
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Reference: | M00434 |
Name: | Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody |
Category: | Antibody |
Subcategory: | Primary Antibodies,Rabbit Monoclonal Antibodies |
Research Area: | DNA/RNA,DNA Synthesis,Epigenetics and Nuclear Signaling |
Aditional Names: | DNA topoisomerase 1;5.99.1.2;DNA topoisomerase I;TOP1; |
Unit: | 30ul 100 ?l |
Concentration: | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
Clonality: | Monoclonal |
Price: | Consultar |
Format: | Liquid |
Immunogen: | A synthesized peptide derived from human TOP1 |
Host: | Rabbit |
Reactivity: | Human, Mouse |
Application: | Flow Cytometry,IF,IHC,ICC,WB |
Description: | Boster Bio Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody catalog # M00434. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. |
Function: | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site i |
Cell localization: | Nucleus, nucleolus. Nucleus, nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli. |
Clone: | Clone: FIG-20 |
Isotype: | Rabbit IgG |
Purification: | Affinity-chromatography |
Storage conditions: | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
Assay inforation: | WB 1:1000-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 Flow Cytometry 1:50 |