| Supplier: | Boster |
|---|---|
| Reference: | M00434 |
| Name: | Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody |
| Category: | Antibody |
| Subcategory: | Primary Antibodies,Rabbit Monoclonal Antibodies |
| Research Area: | DNA/RNA,DNA Synthesis,Epigenetics and Nuclear Signaling |
| Aditional Names: | DNA topoisomerase 1;5.99.1.2;DNA topoisomerase I;TOP1; |
| Unit: | 30ul 100 ?l |
| Concentration: | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Clonality: | Monoclonal |
| Price: | Consultar |
| Format: | Liquid |
| Immunogen: | A synthesized peptide derived from human TOP1 |
| Host: | Rabbit |
| Reactivity: | Human, Mouse |
| Application: | Flow Cytometry,IF,IHC,ICC,WB |
| Description: | Boster Bio Anti-TOP1/Topoisomerase I Rabbit Monoclonal Antibody catalog # M00434. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. |
| Function: | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site i |
| Cell localization: | Nucleus, nucleolus. Nucleus, nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli. |
| Clone: | Clone: FIG-20 |
| Isotype: | Rabbit IgG |
| Purification: | Affinity-chromatography |
| Storage conditions: | Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Assay inforation: | WB 1:1000-1:2000 IHC 1:50-1:200 ICC/IF 1:50-1:200 Flow Cytometry 1:50 |